摘要
本文建立的Ｄｏｔ－ＥＬＩＳＡ对猪衣原体ＩｇＧ的最小检测量为１．３３×１０－９ｇ．与间接补体结合试验（ＩＣＦ）比较；检测１１０头份猪血清样品，Ｄｏｔ－ＥＬＩＳＡ检出５２份（４７．２７２％，效价≥６４），ＩＣＦ法检出３２份（２９．０９％，效价≥８）．用异种动物衣原体标准阳性血清作阻断试验，证明Ｄｏｔ－ＥＬＩＳＡ特异性好（阻断可使原效价下降至少８个单位以上），诊断膜与猪瘟阳性血清、猪水疱病阳性血清、猪口蹄疫阳性血清、猪弓形体阳性血清不出现有意义的交叉反应（效价≤１６）．诊断膜片置室温（２５℃左右）、４℃和－２０℃保存１个月后反应效果不变，对照反应均成立，重复性好（重复符合率为９０．９％），反应效果不受不同批次微量反应板的影响，反应板可重复使用多次，试剂用量较常规ＥＬＩＳＡ大大节省，各步骤均可做到严格定量，操作简便（３小时内可完成），不需要特殊检测仪器，结果客观，肉眼易于判断，是微生物和传染病及寄生虫病诊断技术标准化很有前途的新技术之一．
Abstract A Dot-ELISA was developed for detection of Chlamydia antibody in sera of pigs. The minimum amount of purified pig IgG giving positive results(1+, yellow-brown dot) was 1. 33 ng/disc. When comparing the Dot-ELISA tests with the indirect complement fixation tests(ICF) in the sero diagnosis of pig chlymydiosis, 52 of 110 serum samples of pigs (42. 27 % ) were positive at a reciprocal titer of≥64 in Dot-ELISA and 32 of 110 were positive (29. 09% ) at a reciprocal titer of ≥8 in ICP test. It indicated that Dot-ELISA had a good specificity when the reaction was blocked with cattle and duck positive sera and the titer dropped at least 8 units. It had a high coincidece rate (90. 9% ) and the microtiter plates could be used rebately. Tt had been proved to be easy to perform, quick to determine and a new diagnostic technique for pig chlamydiosis.
