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禽脑脊髓炎病毒的分离鉴定 被引量:8

Isolation and Identification of Avian Encephalomyelitis Virus(AEV)
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摘要 通过SPF鸡胚连续、快速传代,从自然发病雏鸡脑组织中分离出一株禽脑脊髓炎病毒.暂命名为AEV-NH937株,该病毒在发病鸡胚的肠上皮细胞、胰岛细胞和大脑神经细胞的胞浆中呈颗粒状,直径25~30nm,对盐酸、氯仿、胰蛋白酶具有抵抗力,在二价镁离子保护下可抵抗热效应(50℃),病毒滴度6.16(即EID(50)=6.16),中和指数245。 Abstract A new strain if AEV, AEV937, was isloated from brain of the naturally diseased nesting chicken by continuouSlly and rapidly passasins in SPF chicken embryos. The virus existed in cytoplasmp of intestinal epithelial cells, pancreas islet cells and cerebral nerve cells of the embryos inoculated with AEV937. The viral P8rticle was hexagonal and with a diameter of 25-30urn. The virus was resistant to acid chloroform, trypsin and heat(50C ) under the protection of two-valent Magnesium ion. The virus titer was 6. 16 and neutralization index was 245.
机构地区 内蒙古农牧学院
出处 《中国畜禽传染病》 CSCD 1995年第2期3-7,共5页
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