摘要
通过采集发病鸡眼部病料等作病原分离培养、形态学观察、生化特性试验，共分离到２株大肠杆菌（９４０１、９４０３）和１株沙门氏菌（９４０２）．９４０１号菌对雏鸡有致病力而对小鼠无致病力；９４０２号菌对雏鸡及小鼠均有致病力．９４０１号首和９４０２号菌对雏鸡的半数致死量分别为４．４０×１０（１０）ＣＦＵ、２．２０×１０（１０）ＣＦＵ；９４０２号菌对小鼠半数致死量为２．１４×１０（１０）ＣＦＵ．交叉凝集反应结果表明，９２０１、９２０２、９２０３、９３０１、９３０２、９３０３号菌属同一血清型；９３０４、９３０５、９３０６、９４０１、９４０３号菌分属不同血清型。
Abstract Three gram--negative bacteria were isolated from 'the eyes and livers of sick and dead birds suffering from the ophthalrnitis distributed in Chongqing. They were classfied as Escherichia colt (2 isolates) and salmonella pullorum (1 isolate). Two of the three isolates of gram-nesative bacteria were inoculated respectively intraperitoneally into small white mice and 7-days-old male yisa chickens. The results indicated that E. colt is hishly virul:ent in 7-day-old chickens with a mean lethal dose (LD5o ) of 4. 4 × 10loCFU, S. pullorum is respectively highly virulent in small white mice and 7-da-old chickens with LDso of 2. 14 × 1010 CFU and 2. 20 × 1010 CFU.
