摘要
通过隔玻片ＳＤ大鼠瓦勒氏变性远段坐骨神经获得的雪旺氏细胞（ＳＣ）与胎龄１４天的ＳＤ大鼠胚胎脊髓前角神经元（ＳＡＨＮ）联合培养，用倒置显微镜、Ｎｉｓｓｌ染色、ＳＡＨＮ免疫学观察，证实培养中的ＳＣ具有维持神经元成活和促进突起生长的作用。在这二种神经营养因子作用下，脊髓前角神经元胞体增大，穷起生长可达数倍胞体长度。为认识和研究运用神经营养学说促进周围神经再生提供了新资料。
chwann′s cells were obtained from the distal end of the sciatic nerve follow ing Waileriandegeneration of SD rats. These cells were cultured with the anterior horn neuron of spinai cord of14-day-old SD rat fetus,The two kinds of cells were separated by a slice,Through the micro-scope,the dendrites and the morphology changes at the 24th, 48th,72th, and 96 th hour aftercuIture were observed.It was demonstrated that the Schwann′s cells played the role of maintain-ing the survival of neuron and promoting the growth of dendrites.It was said that theSchwann′s cells could secrete neurot rophic factor which made the body enlarged and caused thedendrites enlonged to several times of the body.
出处
《中国修复重建外科杂志》
CAS
CSCD
1995年第4期226-228,共3页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
神经元
神经再生
雪旺氏细胞
神经营养因子
Schwann′s cell
Neuron
Neurotrophic factor
Regeneration of periphral nerve.
