摘要
将２８只犬的股静脉移植于同侧股动脉，术前分别作４０ｋＰａ、８０ｋＰａ和１２０ｋＰａ液压扩张，设立对照组（非扩张）。术后不同时间取材，作ｐｏｌｌａｋ染色，利用微机图像分析系统形态定量学方法测定移植静脉弹力纤维、胶原纤维及平滑肌纤维相对含量。结果发现：移植静脉弹力纤维术后４周显著下降（Ｐ＜０．０１），４～１６周维持稳定，４０ｋＰａ和８０ｋＰａ扩张组与对照组相差不显著（Ｐ＞０．０５），但１２０ｋｐａ扩张１６周时仍呈下降趋势。胶原纤维含量基本维持不变。血管刚度值（Ｃ／Ｅ）随时间延长而增加，但以１２０ｋｐａ组增加显著。平滑肌含量与术后时间成正比，术后１周与压力成反比，但在术后１６周与压力成正比，其变化趋势与内膜增生相仿。结果说明：４０ｋＰａ和８０ｋＰａ压力扩张对移植静脉弹力纤维含量及刚度值影响不大，但１２０ｋＰａ扩张对移植静脉弹力纤维，平滑肌纤维及刚度值影响很大；压力扩张可能是导致移植静脉内膜过度增生的重要原因，但对移植早期通畅率无明显影响。
he femoral veins were excised from 28 dogs and distended with
pressure of 40, 80 and 120kPa,respectively before grafted to femoral arteries. The veins were
harvested at different timesand Pollak sections、were prepared which revealed different stains
of elastin,collagen and smoothmuscle in each section. The sections were led to image analysis
systerm to computerize the relativecontents of the above components.The results were as
follows EIilstin decreased significantly at4weeks(P<0. 01)and was constant between 4 and 16
weeks.No statistical difference wasfound in 40, 80 kPa and the control group(P>0. 05),but the
elastin of 120 kPa group by the16th week was still decreasing. Collagen of each group had no
difference, but C/E increased sig-nificantly with time。 Smooth muscle contents were correlated
positively with time, and negative-ly with the pressure at 1 week, then positively with the
pressure at 16th week. The changes ofthe above trends were the same as development of
intimal hvperplasia. The contentions were theValue of C/E was determined by the arterial
pressure but that of 1 20 kPa pressure was morehigher.The preim plant pressure diste nsion
was a possible sig nificant factor leading to excessiveintimai hyperplasia of early and middle
stage of aut og enous vein grafts.
出处
《中国修复重建外科杂志》
CAS
CSCD
1995年第2期72-75,共4页
Chinese Journal of Reparative and Reconstructive Surgery
