摘要
在克隆了人ＬＰＬ基因５′端、３′端和基因间调控序列的基础上，构建了多种含ＣＡＴ报告基因的表达重组子。经对人ＬＰＬ不同缺失变异型启动子功能的体内、外分析，发现在５′端序列中存在着与组织特异性表达和分化有关的Ｌｐ－α、Ｌｐ－β，以及位于—７０２、—４８６、—６６６和—４３０区的调控结构。转基因小鼠体内的ｐＨＬＰＬ４．０＋ｉｎｔｒｏｎ和ｐＨＬＰＬ４．０＋３′，不仅呈现了很强的启动活性，而且对—４．０ｋｂ序列中的负调节区具有极好的抑制作用。
The 5’-and 3’-flanking as well as intragenic sequences of human LPL gene werecloned.Many vectors that express a chloramphenicol acetyltransferase(CAT)reporter gene driven byabove regulatory regions were then constructed.When the reporter gene constructs with different pro-moter deletion mutants were introduced into 3T3-F442A cells and mice,we could delimit two Cis-regu-latory elements,Lp-αand Lp-βin5’-flanking sequence that are associated with tissue specific expres-sion and preadipocyte differentiation.In addition,several regulatory sequences, such as 702, 486, 666 and 430 regions were also identified.Both pHLPL4.0+intron and pHLPL4.0+3’constructsin transgenic mice are not only able to strongly induce the production of CAT,but to efficiently sup-press negative regulatory elements in 4.0 kb5’ flanking sequence of human LPL gene.
出处
《中国兽医学报》
CAS
CSCD
1995年第1期10-15,共6页
Chinese Journal of Veterinary Science
基金
美国Oklahoma医学研究基金会
