摘要
本实验用丙基硫脲嘧啶造成鸡胚发育处在甲状腺激素合成不足的状态下,以~3H-秋水仙碱为探针,连续测定不同胚龄的鸡胚脑微管蛋白的含量。结果表明,甲状腺激素缺乏组脑微管蛋白含量明显低于对照组,而脑总蛋白含量两组间差异无显著性。说明甲状腺激素特异性地促进脑微管蛋白的生物合成。
The thyroid hormone deficiency was made in chick embryos by injecting propyl-thiouracil (PTU). The tubulin
was quantitated by 3H-colchicinc assay (expressed as colchicine binding activity). Thyroid hormone deficiency resulted
in a 16% to 28% decline of the tubulin level in the developing chick brain from 15 to 19 days of embryonic age as compared with the control, whereas the total protein amount was not significantly affected.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1989年第2期100-102,共3页
Chinese Journal of Endocrinology and Metabolism
