摘要
１９９３年６～８月间，在河北省涿鹿县捕获部分蜱类，经分离与检测，从全沟硬蜱中肠分离出１株莱姆病螺旋体，以ＰＣＲ法从１只全沟硬蜱成蜱及１只日本血蜱若蜱体内检出莱姆病螺旋体ＤＮＡ阳性，从日本血蜱检出莱姆病螺旋体在国内外尚属首次。此外，我们还以ＩＦＡ、ＥＬＩＳＡ和Ｗｅｓｔｅｒｎｂｌｏｔ３种方法对５名可疑莱姆病患者血清进行了检测，１份血清ＩＦＡ试验ＩｇＧ抗体阳性，５份血清在Ｗｅｓｔｅｒｎｂｏｌｔ试验中出现的蛋白带有１～８条不等。
One hundred and fourty～seven ticks were collected in Zhuolu county on 30 June and on 24 August,1993.Out of these ticks,63 were Ixodes persulcatus, 83 were Haemaphysalis japonicum and l was Dermacentor silvarum,l strain of Borrelia burgdorferi was isolated from 34 Ixodes persulcatus,25 ticks were completed by PCR,the results showed that 1 Ixodes persulcatus adult and 1 Haemaphysalis japonicum nymph were Borrelia burgdorferi DNA positive. To our knowledge,It was the first time that Borrelia burgdorferi was recovered from Haemaphysalis japonicum by PCR. In addation, 5 sera sampled from 5 patients with suspected Lyme disaese were tested with IFA,ELISA,and Western blot.The Western blot results showed l-8 different protein bands.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1995年第3期22-25,共4页
Chinese Journal of Zoonoses
