摘要
已构建的ＣＤ２３ｃＤＮＡ全基因克隆ｐＢＣＤ９７６，以ＥｃｏＲＩ和ＢａｍＨＩ双酶切，回收ＣＤ２３全基因，定向插入ｐＵＣ１８载体，构建ｐＵＣ１８－ＣＤ２３全基因重组体（ｐＵＣＤ９７６）。进一步利用该基因中第３９９位的ＨｉｎｄⅡ酶切点，在ｐＵＣ１８中构建了ＣＤ２３Ｎ端４０３ｂｐ和Ｃ端６０７ｂｐ的二个亚克隆，分别称为ｐＵＣＤ４０３Ｎ和ｐＵＣＤ６０７Ｃ。并对ｐＵＣＤ６０７Ｃ进行测序，最终确认其为ＣＤ２３基因。这将为ＣＤ２３的进一步深入研究提供可靠的物质基础。
The fragment cut with
EcoRI and BamHI from the recombinant plasmid pBCD976 was in-serted
into pUC18 vector,constructing a new recombinant
plasmid-pUCD976.Then,the plas-mid pUCD976 was cleaved with HindⅢ.The
Hind fragment(607bp)was recovered andcloned into pUC18,The
recombinant plasmid pUCD607C was selected and sequenced.The
resultshowed that the fragment cloned in pUCD607C was human CD23
cDNA.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1995年第6期340-342,共3页
Chinese Journal of Immunology
基金
国家自然科学基金
