摘要
用ＰＣＲ技术从我国水稻品种“中花１０ 号”（Ｏｒｙｚａ ｓａｔｉｖａ ｖａｒ． ｊａｐｏｎｉｃａ ｃｖ． Ｚｈｏｎｇｈｕａ １０）的未成熟种子中克隆到ＡＤＰ－葡萄糖焦磷酸化酶基因，进行了全序列分析，并与国外已报道的同类基因进行了核苷酸及推导氨基酸序列的同源性比较。结果表明，作者克隆的ＡＤＰ－葡萄糖焦磷酸化酶基因全长１４６１ ｂｐ，编码１个由４８３ 个氨基酸组成的多肽，与国外基因的核苷酸和推导氨基酸同源率分别为９９．６％ 和９９．７％ 。此外，还对该基因进行了结构及进化分析。
ADP glucose pyrophosphorylase is a key enzyme in the pathway of starch synthesis. The gene encoding this important enzyme was cloned by PCR amplification from immature seeds of a Chinese rice cultivar Oryza sativa var. japonica cv. Zhonghua 10. It was then sequenced and compared with the same gene reported from other rice cultivar. The gene obtained in this study is composed of 1461 bp and encodes 483 amino acid residues. Its nucleotide sequence and deduced amino acid sequence shared 99 6% and 99 7% homology with those of published data respectively. The analyses on the structure and evolution of this gene have been conducted.
基金
"863"及国家自然科学基金
