摘要
1992年春，四种菌核病接种方法的比较试验表明，带菌丝麦粒无伤口接种方法效果最好：致病能力强（平均发病等级1．67），并有利于区别品种间的抗（感）病差别（均差0．64）；病毒病接种方法以1∶10磷酸缓冲液（0．5M，PH7．4并含0．1％Na2SO3）于幼苗3～5叶期效果最好。1993～1994年，用上述两种方法对浙江省140份甘蓝型油菜品种资源的抗性鉴定表明：对菌核病抗病的材料1份、中抗9份、中感23份、感病50份、高感36份；对病毒病无抗病以上材料，中抗10份、中感55份、感病35份、高感9份。
The infectional experiments were conducted to determine the most practical methods to inoculatepathogens in spring 1992. It was demonstrated that binding infected barly kennel with stem was a desirablernethod to inoculate S.sclerotiorum in terms of the effectiveness (DI 1 .67) and the wider diversity(0.64) among varieties. The method, rubbing leaves with TUMV carried emery in the solution (0.5M PH7.4) of 1: 10 phosphatide acid and 1 % Na2SO3 at 3~ 5 leaves stage, was selected to incoculate virus. 140rapeseed genetic materials in Zhejiang had been evaluated during 1993~ 1994. I resistant, 9mid-resistant, 23 mid-sensible, 50 sensible and 36 high-sensible materials t0 S. sclerotirum and 10mid-resistant, 55 mid-sensible, 35 sensible and 9 high-sensible materials to virus exist in the germplasm.
出处
《浙江农业学报》
CSCD
1995年第3期202-205,共4页
Acta Agriculturae Zhejiangensis
关键词
甘蓝型油菜
菌核病
病毒病
鉴定
抗病性
浙江
Brassica napus L.
Sclerotinia sclerotiorum
Virus disease
Resistance evaluation
