摘要
人工低压舱内模拟高原低氧24h,并与2300m对照组比较,观察不同发育年龄大鼠SGOT活力,肝溶酶体总酸性磷酸酶、非沉淀酸性磷酸酶和芳基硫酸酯酶活力及肝重、肝细胞糖原、蛋白和总脂含量的变化。在海拔5000m高度,10天鼠各酶活力、570天鼠总酸性磷酸酶和芳基硫酸酯酶活力明显升高;35和75天鼠各酶活力未见显著变化;在海拔8000m高度,各年龄组鼠上述各酶活力均显著升高。随着海拔高度的升高,各组大鼠肝重呈不同程度的下降,肝细胞糖原含量非常明显地减少,35和75天鼠8000m组全肝蛋白含量下降明显,10、35、75天鼠肝细胞总脂累积。上述结果综合分析表明:低氧致使大鼠肝细胞损伤属一普遍性效应,新生期和老年期大鼠肝细胞耐低氧能力不及幼年期和成年期大鼠。
This paper reported the effect of hypoxia on liver cells and the lysosomes in rats at various developmental ages. The rats of 10, 35, 75 and 570 days old were used in present study.The result showed that after exposure to 8 000m of altitude in the hypobaric chamber for 24h, activities of SGOT, total and nonsedimentary acid phosphatase and aryl sulphatase enhanced significantly in rats of various ages. At 5 000m of altitude, all of these indices measured in 10 days rats, total acid phosphatase and aryl sulphatase in 570days rats increased markedly and no change of the indices was showed in 35 and 75 days rats at 5 000m of altitude (table 1, figure 1-3). These phenomena indicated that hypoxic damages of liver cells ocured universally in rats, and the adaptation to hypoxia was unsufficient in neonatal and aged rats.Hepatic glycogen contents decreased seriously with the increase of altitude elevation. Protein levels(mg/ g liver) increased markedly only in 35 days rats, whereas it declined markedly in calculating by mg / whole liver in 35 and 75 days rats whose liver weight droped severely vs neonatal and aged rats at 8 000m of altitude(table 2-4).The accumlation of total lipid of livers appeared in general except the aged rats during hypoxia (table 5). The mechanism might be an inhibition of β-oxidation process in liver mitochondria of rats under hypoxic conditions.
出处
《中国应用生理学杂志》
CAS
CSCD
1989年第1期15-21,共7页
Chinese Journal of Applied Physiology
基金
国家自然科学基金3860781
关键词
低氧
肝细胞
溶酶体
hypoxia
hepatic lysosomal enzymes
glycogen
protein
total lipid
