摘要
T2毒素2.3 mg/kg(相当LD_(30))单次ip后测定小鼠外周血象,BMNC及骨髓CFUs、CFU—GM、CFU—E和BFU—E的改变以了解T2毒素血液毒理的特征.外周血中WBC总数明显减少,RBC和pt无变化.T2毒素虽不影响CFUs的数量,但对CFU—GM、CFU—E和BFU—E均有杀伤作用,中毒后2 h三种细胞存活率达最低值分别为正常的27％、48％和57％,恢复速度甚快,5 d内全部正常.三种细胞的T2毒素剂量活存曲线均为指数坪型.分析了各类造血干细胞对T2毒素敏感性不同的原因可能与各种细胞固有特性和细胞增殖状态有关。
T2 toxin is one of the most toxic member among trichotheccnes (Ts)family produced by fungi.It is responsible to intoxication found in human being and animals following ingestion of mouldy grain,and alimentary toxic aleukia (ATA)is the common but important problem in intoxication.The follows are some events in hcmotological toxicology of this toxin.Under a single dose (2.3mg/ kg ip,equal to LD30),T2 toxin decreased mice peripheral WBC count and a similar decline of circulating lymphocyte (L)also happened,but an earlier transient increase of both WBC and L at 3 h following injection was observable.The nadir of WBC was 38% on d 1 and recovered to normal by d 7.The transient increase of polymorphonuclear neutrophil count maintained for 6 h then decreased gradually to its nadir on d 3 and returned to normal level by d 7.RBC and pt were uneffectcd.By determining CFU-S,CFU-GM,CPU-E and BFU-E of femoral bone marrow cells in mice,there were no killing effect for CFU-S during 0.5- 24 h after exposure to the toxin,but a maximal depression ofCFU-GM,CFU-E and BFU-E all were happened at 2 h,with a survival fraction by 27%,48% and 57% respectively.At 0.5 h following administration,CFU-GM decreased about 42% of its control,but CFU-E and BFU-E were 10- 20% lower only.So,the sensitivity of these three committed progenitor cells to T2 toxin was in the order CFU-GM>CFU-E>BFU-E.The recovery of CPU-GM was rapidly and with a rebound value 2-3 times higher than control at d 2 and d 3,and reached normal level by d 5.CFU-E and BFU-E regenerated gradually and recovered by d 3.By detecting at 2 h after intoxication,the dose-response curves for CFU-GM,CFU-E and BFU-E were all appeared the characteristics of exponential plateau pattern.The sensitivity of hemopoietic stem and progenitor cells to T2 toxin in concerning with the character of each cell population and its proliferative manner was discussed.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
1989年第2期144-148,共5页
Chinese Journal of Pharmacology and Toxicology
关键词
T2毒素
造血干细胞
祖细胞
T2 toxin
hemopoieticstem cells
colony forming unit
dose-response relationship
