摘要
酵母多糖刺激[~3H]AA预标的小鼠腹腔巨噬细胞可释放[~3H]AA代谢物LTC_4,LTB_4,TXB_2,6-keto-PGF_(1α)及PGE_2.山莨菪碱可显著抑制上述刺激释放作用,使总~3H释放量及各[~3H]AA代谢物释放量均显著减少.这种抑制作用随山莨菪碱剂量增加而增强.当其浓度为0.5 mmol/L时.总~3H释放量下降46％(2 h),45％(5 h)及34％(15 h).本实验结果进一步证实山莨菪碱是通过抑制AA释放而减少PG及LT的形成.
[3H]arachidonic acid (AA)prelabeled mouse peritoneal macrophageswere stimulated by zymosan to release [3H]AA metabolites.The major labeled products which were co-chromatographed with the authentic PG and LT standareds by TLC and determined by liquid scintillation were LTC4,LTB4,TXB2,6-keto-PGF1α and PGE2 Aniso-daminc (Ani)significantly inhibited the zymosan-induced release of PG and LT from mouse,macrophages in a dose-dependent manner.Both the total 3H and each of AA metabolites released in media reduced in the presence of Ani.At 0.5 mmol / L of Ani,the zymosan-induced release of total 3H was reduced by 46%(2 h),45%(5 h),and 34%(15 h),respectively.The results of thepresent study further confirmed the hypothesis that Ani decreased the formation of PG and LT via the inhibiting release of AA from the cell membrane.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
1989年第2期91-95,共5页
Chinese Journal of Pharmacology and Toxicology
基金
国家自然科学基金
关键词
山茛菪碱
酵母多糖
巨噬细胞
anisodamine
arachi-donic acids
prostaglandins
leukotriencs
macrophages
zymosan
