摘要
研究了红花对蒙古沙土鼠脑缺血和再灌注时４００００ｒ／ｍｉｎ离心大脑皮质可溶住Ｃａ２＋／ＣａＭ－ＰＫⅡ活性的影响。实验结果如下：（１）脑缺血１０ｍｉｎ组酶活性为假手组的１３．６％；而缺血前给药组（６．７ｇ红花／ｋｇ体重）酶活性为假手组的７９．４％。（２）红花拮抗脑缺血诱导该酶活性的抑制具有剂量依赖性。（３）脑缺血１０ｍｉｎ后给生理盐水再灌注２ｈ，酶活性恢复至假手术组的９２％，而缺血后给药再灌注不能加速酶活性的恢复。
The effects of Carthamus tinctorius L.on the activity of Ca2+/CaM-PKⅡ was investigated in the 40000r/min centrifugated cytosolic fraction of cerebral cortex from Mongolian gerbils having undergone transient cerebral ischemia and reperfusion. The results were as follows;(1)The activity of Ca2+/CaM-PKⅡ in 10-min cerebral ischemia group was 13.6%that of sham group;the activity of the enzyme in the test group(with drug 6.7g/kg body weight administered 20 min prior to 10-min cerebral ischemia ) was 79.4%*at 0f sham group.(2)The inhibition of the enzyme activity induced by cerebral ischemia was antagonized by Carthamus tinctorius L.and the antagonism was dose-dependent.(3)After 10min of cerebral ischemia and 2h of reperfusion,the enzyme activity of the NS ip control group was 92%that of sham group,the recovery of the enzyme activity was not further improved by giving Carthamus tinctorius L. after ischemia.
出处
《徐州医学院学报》
CAS
1995年第3期225-228,共4页
Acta Academiae Medicinae Xuzhou
基金
国家自然科学基金
关键词
脑缺血
再灌注
红花
蒙古沙土鼠
钙离子
cerebral ischemia and reperfusion
Ca2+/CaM-pKⅡ
Carthamus tinctorius L.
Mongolian gerbil
