摘要
目的探讨ＰＣＲ—ＴＢ－ＤＮＡ在结核性胸膜炎早期诊断的临床价值。方法应用聚合酶链反应（ＰＣＲ）技术对４８例结胸胸水进行结核菌ＤＮＡ检测，同时检测胸水结核菌、结核抗体及皮肤ＰＰＨ试验，并设２６例非结核性胸水作对照组，进行上述指标测定。结果４８例结核性胸水涂片抗酸染色、皮肤ＰＰＤ试验、结核抗体和ＰＣＲ检测阳性率分别为８．３３％、２０．８３％、８１．２５Ｍ和６８．７５％，后二者显著高于前二者（Ｐ均＜０．０１），而且，ＰＣＲ阳性结果出现早于结核抗体。结论ＰＣＲ对结胸胸水有早期病原学诊断价值。
Objective The purpose of this article was to research the clinical value of PCR for TB-DNA in early diagnosis of tuberculous pleural effusion.Method Polymerase chain reaction was applied to detect DNA of my cobacterium tuber culosis in 48 patient with tuberculous plearisy. It also was applied that determine of mycobacterium tuberculosis of pleural effusion and anti-tuber culosis and PPD skin test in 48 patients with plearal effusion. It was designed that 26 patients with non-tuberculous effusion served as control group.Results It was found that the positive rates of acid-fast staining of pleural effusion, PPD-skin assay, anti-tuberculosis add PCR in 48 patieats with tuberculous plearisy were 8. 33%, 20. 83%, 81. 25% and 68. 75%, respectively. The sensitivity of PCR and anti-tuberculosis were much higher that those of acid- fast staining and PPD-skin test (all P< 0.01) .Furthermore, PCR positive result was earlier appearance than anti-tuberculosis that.Conclusion PCR technigue has the earlier diagnosis value for exudative tuberculous pleurisy effusion.
出处
《新乡医学院学报》
CAS
1995年第4期350-352,共3页
Journal of Xinxiang Medical University
关键词
聚合酶链反应
结核性胸膜炎
结核
胸水
诊断
polymerase chain reaction
tuberculosis
exudative tuberclous pleurisy
