摘要
癌基因erbB-2CR_2片段的扩增与克隆周红,孙亚萍,郑耘,林炳珍,杨善民,汪德耀(抗癌研究中心)癌基因erbB-2在细胞内编码与人表皮生长因子受体EGFr结构相似的转膜蛋白P ̄185,该P ̄185蛋白的过量表达具有强的转化作用,参与乳腺癌、胃癌及...
A DNA sequence(CR_2 )of 561 bpsizefrom oncogene erbB-2 was amplifiedby PCR reaction and purified.This DNA fragment was then linked with pLW_1 to construct a re-combinant which was transformed into E. Coli DH5a,By means of dot blot,five transformantswere convinced of carrying the CR_2 sequence.
出处
《厦门大学学报(自然科学版)》
CAS
CSCD
北大核心
1995年第3期479-482,共4页
Journal of Xiamen University:Natural Science