摘要
以香菇（Ｌｅｎｔｉｎｕｓｅｄｏｄｅｓ）种内不同株一对亲和的单核菌丝（７４０２〈２〉和９１０１〈１２〉）为亲本，以碘乙酰胺失活７４０２〈２〉作为筛选标记，经过原生质体制备、ＰＥＧ融合及融合子再生等步骤，选育得融合子。融合子与双亲无拮抗性，在菌丝形态，核数目及可溶性蛋白质图谱、酯酶同工酶谱和过氧化物酶谱上均与双亲单核菌丝及担孢子杂交子有区别。将Ｋｎｏｗｌｔｏｎ等于１９８４年建立的一种分离高频再生衍生株的方法首次运用至食用菌中，使原生质体再生率提高２－３倍，为融合操作提供了方便。
An intraspecific fusant of L. edodes(Fu2)was obtained by fusing the protoplastsprepared from the monokarvotic mvcelial strains 7402(2)and 9101(12),with the strain 7402(2)having an inactivation mark obtained by CIA treatment.The fusant was identified byexamining the mycelial morphology and the electrophoretogram of soluble proteins and esterase/catalase isozymes。It showed no antagonistic action against both of the parent strains 7402(2)and9101(12).On plate it grew faster than the monokaryotic parents。The fusant differed in mycelialmorphology,nucleus number/per cell and the electrophoretogram of soluble proteins andesterase/cataiase isozymes from the parents and the basidiospore heterozygote,In this study,Wehave,for the first time,successfully applied the method developed by Knowlton et al.forimproving regeneration-frequency to edible mushroom breeding。By using this method we obtainedaregeneration strain of R_3,its regeneration frequency is 2-3times that of monokaryoticmvcelium 9101(12)。
出处
《微生物学通报》
CAS
CSCD
北大核心
1995年第2期67-71,共5页
Microbiology China
关键词
香菇
融合子
原生质体融合
食用菌
Lentinus edodes,Protoplast,Fusant,Isozyrne,Electrophoretic analysis
