摘要
本文对混合单克隆抗体ELISA直接法和ELISA夹心法检测包虫病人循环抗原的效果进行了比较研究。结果表明,ELISA夹心法对细粒棘球蚴囊液纯化抗原的最低检出量(1.2ng/ml)较直接法(10ng/ml)低,但检测包虫病人循环抗原时,ELISA直接法的阳性检出率为56.39%(27/48),ELISA夹心法为60.4%(29/48),二者无显著性差异(P>0.05)。两法同时检测14份猪囊尾蚴病人血清和44份正常人血清,均未出现假阳性反应。
The circulating antigen in patients with hydatid disease was detected by Direct-ELISA and Sandwich-ELISA using a mixture of monoclonal antibodies.The results showed that the minimal detectable level of Echinococcus granulosus cyctic fluid antigen(EgCFAg)by Sandwich-ELISA(1.2ng/ml)was lower than that by Direct-ELISA(10ng/ml),but for detecting circulatingantigen(CAg)in patients sera,the CAg positive rate of the Sandwich-ELISA was 60.4%(29/48)and 56.3%(27/48)in the Direct-ELISA,it was no significant difference(p>0.05).14 samples of patients with human cycticercosis and 44 samples of normal sera were tested by Direct-ELISA and Sandwich-ELISA at the same time.The results showed that there were not falsepositive reaction in these two methods.
作者
哈小琴
景涛
曹和洵
Ha Xiaoqing(Department of Urology,Generaal Hospital of PLA,Lanzhou 730050)
出处
《微生物学免疫学进展》
1995年第3期150-154,共5页
Progress In Microbiology and Immunology
关键词
包虫病
循环抗原
单克隆抗体
ELISA
Hydatid diseasei
Circulating antigen
Monoclonal antibody
Direct—ELISA
Sandwich—ELISA
