摘要
中国板栗(Castanea mollissima Blume)对板栗疫病菌(Cryphonectria parasitica(Murr.)Barr)具有强的抗病力。但近十多年来,我国许多省份均发现有板栗疫病,据调查,广西有19个县市存在栗疫病,有的地方发病还相当严重。为了解释疫病发生的上述情况,并为生产和该病菌的更深入研究提供指导,作者在广西桂林、南宁、柳州、梧州、河池等地收集菌株,对疫病菌致病性的分化进行了研究,现将结果报道如下。 1 材料和方法 1.1 毒力参照菌株 法国已知弱毒株EPF为毒力参照株。 1.2 栗疫病菌的采集、分离和单孢纯化 1.2.1 标本采集:1988年12月～1989年6月,采自广西桂林、南宁、柳州、梧州、河池等地板栗病株。 1.2.2 菌株分离和纯化:将病枝或病树皮,用经灯焰灼烧过的刀片削去表皮,取坏死内皮约5×5mm^2,压于PDA上,28℃,光照培养,产孢后用微块法进行单孢纯化。 1.3
According to the results of inoculations on seven Chinese chestnut varieties with nine selected strains of chestnut blight fungus obtained from five regions in Guangxi the strains could be divided into supervirulent strains, virulent strains and hypo-virulent strains, but they had no differentiation of physiologic races. The virulence in chestnut varied with different virulence types. If the mycelia of a hypo-virulent strain was mixed with the mycelia of a supervirulent strain, the later virulence could be weakened greatly. All of nine tested strains contained fungal virus RNA (dsRNA), it proved that the hypo-virulence was not controlled by dsRNA. Most Chinese chestnut varieties had high resistance to chestnut blight fugus, although there were some differences among these varieties.
出处
《微生物学报》
CAS
CSCD
北大核心
1995年第6期470-473,共4页
Acta Microbiologica Sinica
关键词
板栗疫病菌
致病力
分化
板栗疫病
Chinese chestnut, Chestnut blight fungus, Strain, Virulence differentiation
