摘要
对多聚酶链反应（ＰＣＲ）扩增条件相同情况下，５种标本处理方法ＨＢＶＤＮＡ检测结果进行了比较。结果证实，酶消化法、碱变性法和微波法ＨＢＶＤＮＡ检出率（均为７１．８８％，４６／６４）高于直接法和热变性法（均为６２．５％，４０／６４），而且酶消化法、碱变性法和微波法的敏感性明显高于直接法和热变性法。综合比较这５种ＰＣＲ方法，碱变性法和微波法不仅敏感性高、简便和快速，而且抗污染性强、结果稳定可靠，值得推广应用。
Under the same amplification condition of polymerase chain reaction (PCR),five different sample treatment methods for detection of HBV DNA were compared.The results showed that 71.88%(46/64) of samples was detected HBV DNA positive by enzyme digestion,NaOH denaturation and microwave methods,while only 62.5%(40/64) was detected HBV DNA positive by direct detection and heat denaturation methods,We demonstrated that enzyme digestion,NaOH denaturation and microwave methods were more sensitive than direct detection and heat denaturation methods.Compared with other methods,NaOH denaturation and microwave methods were not only sensitive,simple and quick,but also contamination-free and reliable.
出处
《同济医科大学学报》
CSCD
北大核心
1995年第3期181-183,共3页
Acta Universitatis Medicinae Tongji
