摘要
本文采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和抗原印渍(Western blotting)技术对钩端螺旋体赖型70091株的两种抗原成份,即红细胞致敏物(ESS)和脂多糖(L-LPS),作了抗原分析。提示ESS保护性抗原决定簇位于相当标准分子量蛋白的23kd处;结合化学组成分析,认为这一片段以多糖为主。L-LPS在同一位置似乎也有共同抗原。
This paper reports the antigenic analyses of two antigens from Leptos-pira interrogans serovar iai strain 70C91 i.e. leptospiral erythrocyte sensitizing substance (ESS) and lipopolysaccharide ( LPS ) with the methods of SDS-PAGE and Western blotting. The results showed tnat a band of ESS at approximately 23 Kd was possibly a fraction of antigenic determinants responsible for the protective immunity and the fraction might be mainly composed of sugar.The LPS seemed to have a common antigen at the same position.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1989年第2期2-4,共3页
Chinese Journal of Zoonoses
