摘要
EB病毒在流行病学上与鼻咽癌癌变密切有关。 应用体外转染法,将EBV—W(YH)片段导入RatI细胞,在20天时出现细胞交叉重叠生长的转化灶。经软琼脂生长筛选后,用分子杂交方法可在转化细胞DNA内检测到W片段的存在,转化细胞接种裸鼠后可生长肿瘤,并在肿瘤组织DNA中再次检测到EBV—W(YH)片段的存在,转化细胞染色体的原位杂交分析提示,在有些中期相染色体上有银粒出现。 以上结果说明,EBV—W(YH)基因片段已转染至细胞内,并能在大鼠细胞内复制,与肿瘤形成呈正相关,它可能对细胞的依赖生长起着重要作用。
The association of EBV with poorly differentiated or anaplastic NPc is virtually confirmed.Rat-1 cells were transfected with EBV-W(YH) (BamH1) fragment by calciutr phosphate precipitation method. On the 20th day after transfection, transformed foci appeared that were composed of highly refractile, multilayered morphologically altered cells. These foci were cloned in soft agar. Colonies were recovered and have been picked up Foci DNA was digested by BamH, and then hybridized with EBV-W(YH) DNA. The signal could be detected in transformed foci DNA.The cloned foci was inoculated into the nude mice and subcutaneous tumor was formed. The EBV-W(YH) fragment in the transplanted tumor DNA has been detected.In situ hybridization of the chromosome from transformed rat-1 cells showed silver granul-les in mataphase cells.These results indicated that the transforming gene from EBV-W(YH)has been incorporated into and replicated in Rat-1 cells accompanying by tumor formation .
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1989年第5期338-341,共4页
Chinese Journal of Cancer
