摘要
白细胞介素2(IL-2)产生的机理较为复杂,还未澄清。1980年Smith提出有关IL—2产生的两个信号模式面,其中之一为巨噬细胞释放IL-1刺激T细胞产生IL-2,认为IL-2产生必须有巨噬细胞参与。但近年来对巨噬细胞在IL-2产生的需要性提出异议。本文取材扁桃体,在同一实验中探讨巨噬细胞在IL-2产生的需要性的不同观点,以阐明淋巴系统中IL-2产生的机理。
The effect of macrophage on IL-2 production by tonsillar T cells was investigated. The human tonsillar mononuclear cells were purified by a two-step procedure including depletion of plastic-adherent cel1s and rosetting with SRBC. The purity of Enriched T cel1s (ETC) was 98%. It was found that IL-2 production was greatly decreased in ETC by removing adherent cells and could be completely restored by replacing the adherent cells. The IL-2 production in the same extent was induced in ETC when TPA was substituted for adherent cells and the synergistic effect of TPA and macrophages greatly augmented the IL-2 secretion by ETC. Incubation of ETC with high concentration of PHA or ConA alone resultes in efficient IL-2 production. These results indicated that IL-2 production was macrophage dependent.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1989年第5期279-281,共3页
Chinese Journal of Immunology
