摘要
应用ＡＢＣ免疫组化方法和Ｃ－ｅｒｂＢ－２癌基因的单克隆抗体对９３例大肠组织标本进行了免疫组化染色。结果表明：在６８例大肠癌中，Ｃ－ｅｒｂＢ－２阳性表达为６１．８％。其中高分化腺癌组Ｃ－ｅｒｂＢ－２阳性表达为４０％；中分化腺癌组阳性表达为５０％。低分化腺癌组阳性表达为８１．２％；粘液细胞癌组阳性表达为８５．７％。粘液细胞癌组和低分化癌组Ｃ－ｅｒＢ－２阳性表达明显高于其它２组（Ｐ＜０．０１）。在已有淋巴结转移的大肠癌中，Ｃ－ｅｒｂＢ－２癌基因阳性表达为７６．２％（３２／４２）。在无淋巴结转移的大肠癌中，Ｃ－ｅｒｂＢ－２阳性表达为３８．５％（１０／２６）。二者比较，Ｃ－ｅｒｂＢ－２癌基因表达有显著性差异（Ｐ＜０．０１）。在１５例大肠腺瘤标本中，Ｃ－ｅｒｂＢ－２癌基因阳性表达为６．７％。１０例慢性炎症和１０例正常肠粘膜Ｃ－ｅｒｂＢ－２则均为阴性。以上这些结果提示：Ｃ－ｅｒｂＢ－２癌基因产物不仅可视为肿瘤的一种标志，而且与大肠癌的分化程度、淋巴结的转移密切相关，这证明Ｃ－ｅｒｂＢ－２癌基因的检测对大肠癌的病理诊断和预后判断有很高的实用价值。
With the use of ABc immunohistochemical thechnique,the
authors examined the expression of C-erbB-2oncogene ptoduct in 93 specimen from large
intestine.Results showed that among them 68 were large intestinal carcinoma,15were adenoma
and l0 were colitis. Positive expression of C-erbB-2 oncogene product was observed obviously
in large intesti-nal carcinoma and positive rate was 61.8%,C-erbB-2 positive rate was 40%in
well一differentiated adenocarcinoma,50%inmoderatedly-differentiated adenocarcinoma,8l.2%in
pooriy-differentiated adenocarcinoma, 85.7%in mucous cell carcino-ma.The positive expression
in later two groups was markedly higher than in former two groups(P <0.0.C-erbB-2 posi-tive
rate was 76.2%(32/42)in specimen with distant metastasis,38.5%(10/26)in the specimen
without metastasis. Therewas significant difference between two groups(P<0.01).C-erbB-2
positive rate was 6.7%in adenoma. C-erB-2 oncogeneprcduct expression in the colitis (10
cases) and normal mucosa(10 cases)were negative. These results suggested∶ C-erbB-2 not
only can be considered as a tumor marker,but also has close relation with the dif-ferentiation
and metastasis of large intestinal carcinoma,the C-erbB-2oncogene may be of great practical
value in pathologicaldiagnosis and prognosis of large intestinal carcinoma.
出处
《实用癌症杂志》
1995年第4期219-221,共3页
The Practical Journal of Cancer
