摘要
用连续前沿色谱法和断续前沿色谱法测定了七种标准蛋白在疏水色谱填料上的吸附等温线，两种方法之间存在着一定的差别。从流出曲线突跃处斜率测定的不确定性、色谱过程中动力学因素和实验方法本身存在的问题等几个方面探讨了误差的来源，指出了可能解决这一问题的途径。
dsorption isotherms of proteins on the surface of the hydrophobic sorbents were measured by consecu-tive and non-consecutive frontal chromatography.It was found that significant difference exsits between theabove two methods. The difference is attributed to the unreliabilitv in the determination of the slope of theinflexion point curve in frontal chromatography,the kenetic factors in chromatographic process and the prob-lems that intrinsically exsit in the two methods. A suggestion which is possible to solve this problem is alsoraised.
出处
《色谱》
CAS
CSCD
北大核心
1995年第3期174-177,共4页
Chinese Journal of Chromatography
关键词
色谱法
疏水色谱
蛋白质
吸附等温线
frontal chromatogrpahy , hydrophobic interaction chromatography,proteins
