摘要
本文用高效表达载体表达出的融合ＢＩＶ核心蛋白（ＴｒｐＥ－Ｇａｇ）和外膜蛋白（ＴｒｐＥ－Ｅｎｖ）作为ＷｅｓｔｅｒｎＢｌｏｔ检测的抗原，分别对Ⅰ、Ⅱ两口岸进口奶牛及后代进行了流行病学调查，发现用外膜蛋白检测阳性率比核心蛋白阳性率略高，进一步的追踪调查表明核心蛋白的抗体水平在减弱的同时外膜蛋白的抗体有加强的趋势。
With the high-expressed fusion core-protein trpE-Gag and envelope-protein trpE-Env as the detective antigens,the imported cows with their progenies were detected by Western Blot assay. The result shows that the rate of positive result by using TrpE-Env as the detective antigen is higher than that by using TrpE-Gag as the detective antigen. Tracing survey of positive cattle indicates that the Gag-specific antibodies in infected cattles are lossing,in contrast,the level of Env-specific antibodies is strengthening.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
1995年第3期6-10,共5页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
国家教委博士点基金
