摘要
应用抗c－myc原癌基因蛋白（p62c－myc）的单抗，采用免疫组化ABC法对正常胃粘膜、胃粘膜异型增生及胃癌进行标记，发现正常胃粘膜腺体颈部偶见弱阳性表达，而胃粘膜异型增生则有较高的表达率，轻、中、重度异型增生的表达率分别为70％、100％及71．4％，三者之间无显著性意义。早期及进展期胃癌的表达率分别为38．9％和73．3％，两者相比有显著性差异（P＜0．05）。本研究结果提示p62c－myc可能参与正常胃粘膜的增殖过程，与胃癌的发生有一定关系，此基因蛋白的表达有可能作为判断胃粘膜早期癌变及胃癌生物学行为的一个有用指标。
The normal gastric mucosa, gastric mucosal dysplasia and gastric carcinoma were marked by using the monoclonal antibody to c-myc proto-oncogene protein(p62'-',') and us-ing the method of immunohistochemical ABC' The results showed that there was a veryweak positive expression of p62,-',,in the neck region of normal gastric mucosal gland, but arelatively high positive rate was found in dysplasia, and 7O%, 1OO% and 7l. 4% positiverates were found in light, moderate and severe gastric dysplasia respectively. The differenceamong the three was no remarkable significance. The positive rates in early and in advancedcarinomas were 38. 9% and 73. 3% respectively, and there was a significant difference be-tween them (P<o. o5). The resu1ts suggest that c-myc proto-oncogene protein might par-ticipate in the proliferative activity of normal gastric mucosa, and play a certain role in theprocess of carcinogenesis of gastric mucosa. The expression of the proto-oncogene proteinmay be used as a useful index which may judge the early canceration and the biological be-havior of gastric carcinoma.
出处
《洛阳医专学报》
1995年第1期3-6,共4页
Journal of Luoyang Medical College
关键词
C-MYC原癌基因
胃肿瘤
癌前状态
c-myc proto-oncogene
p62^(c-myc)
gsatric carcinoma
gastric precancerous lesions
