摘要
本文用４种鼠抗人白细胞抗原的单克隆抗体（ＭｃＡｂ），采用标记的链霉亲合素生物素技术（ＬＳＡＢ）对９例固定性药疹（ＦＤＥ）患者急性期皮损内浸润的Ｔ细胞及其亚群、Ｂ细胞进行标记。观察到损害内真皮中上部血管周围浸润的细胞中６０％～８０％为Ｔ细胞，其中ＣＤ＿４＋细胞和ＣＤ＿８＋细胞数量大致相等。细胞外移以ＣＤ８＋细胞为主。此外还有１０％～２０％Ｂ细胞。提示ＣＤ＿４＋／ＣＤ＿８＋／细胞在介导ＦＤＥ的炎症反应中起主要作用。
Nin patients with fixed drug eruptions( FDE) were tested immunohistochemically with different mouse antihuman monoclonal antibodies(McAb) to identify T lymphocytes, their phenotypic subsets and B lymphocytes. 60 to 80% of lymphocytes perivascularly infiltrating the superficial dermis were found to bo T cells.CD4-positive cells and CD_8- positive cells were approximately equal among the pan-T-positive cells.However, it appeared that CD_8-positive cells were more active in exocytosis than CD_4-positive cells. In addition,about 10 to 20% dermal infiltration cells were pan-B-positive lymphocytes. These results indicated that T inducer/helper and suppressor/cytotoxic cells might play a major role in mediating the inflammatory reaction in FDE.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
1995年第6期350-352,共3页
Journal of Clinical Dermatology
关键词
药物性皮炎
免疫酶技术
免疫组化
Dermatitis medicamentosa Immunoperoxidase techniques, LSAB technique
