摘要
裂叶悬钩子(Rubus laciniatus W.cv.Thornless Evergreen)是蔷薇科悬钩子属一种重要的小果类植物。我们在以离体叶片诱导不定芽成功的基础上,对其叶片不定芽形态发生过程作了进一步观察。供接种的叶片从基部去掉叶柄,按前文的操作顺序进行灭菌,接种和培养。从培养第3天起,从叶片基部与叶柄接合处切取材料固定,以后每隔2天取样一次。
Leaf explants of Rubus laciniatus were cultured on Nitsch and Nitsch basic medium supplemented with either 6-BA 2 mg/L and NAA 0.1 mg/l or 2,4-D 2.5 mg/l and NAA 0.1 mg/l.Adventitious buds were produced from leaf-like structures. Some ultrastructural observations of cells have shown that the most easy change part of a plastid during the cell differentiation in Rubus leaf explant culture in vitro was lamella system,storage starch and plastogluboli.
基金
中国科学院资助课题
关键词
悬钩子
器官发生
组织学
Rubus laciniatus
Organogenesis
Plastid
Photolamella
Starch grain
Leaf-like structure
