摘要
小麦 Triticum aestivum L.苗在 NO_3^--N 完全营养液中培养比在 NH_4^+-N 完全营养液中培养,它们叶细胞内的硝酸还原酶(NR)即 NO_3-NR 比 NH_4-NR 活性增高了15倍,而它们叶片中的稳定因子(NR_(SF)),即 NO_3-NR_(SF)比 NH_4-NR_(SF)活化 NO_3-NR 的能力仅增加0.2倍,表明 NR 与 NR_(SF)不是依存关系;另外在 NO_3^--N 培养的黄化小麦叶片,及黄化缺氮、缺铝,加(?)的叶片中,所有的 NR_(SF)都十分稳定,并且保持较高活性,但这些叶片中没有测出NR 活性,因而认为,在植物叶细胞中,NR_(SF)不是调节 NR 活性的主要条件。
The purpose of this work is to examine the relation between NR and NR_(SF) in the leaves of the wheat plants under the different environmental conditions.The leaves of wheat plants which were grown in the nutritional solution containing NO_3^-,present 15 times higher of NR activity than that grown in the nutritional solution containing NH_4^+,but NR_(SF),which activated NO_3^-NR of the former increased only 20% as compared with that of the latter,indicating that NR is independent of NR_(SF).In addition,in the yellowing leaves dark grown in NO_3-N solution and those in the same solution containing W with no nitrogen and Mo,the NR_(SF) is quite stable,and keep higher activity,but no NR activity can be determined.So the NR_(SF) is not considered as a major factor to regulate activity of NR in the leaves.
关键词
环境条件
硝酸还原酶
稳定因子
Environmental conditions
Nitrate reductase-stabilizing factor(NP_(SF))
Nicrate redutase(NR)
