摘要
报告了检测人巨细胞病毒（ＨＣＭＶ）的套式聚合酶链反应（ＰＣＲ）法，及其在妇产科临床应用的结果。改进了从临床样品中提取ＤＮＡ的方法，优化了套式ＰＣＲ的条件。测得套式ＰＣＲ的扩增倍数为２．９×１０￣９，套式ＰＣＫ／电泳法的灵敏度为４０个ＨＣＭＶ基因组拷贝。临床应用结果表明，孕妇的ＨＣＭＶ感染率很高，母婴间存在ＨＣＭＶ感染的垂直传播关系。胎盘是传播的主要途径，胎盘的屏障功能随妊娠时间的延长而减弱。宫颈分泌物的感染也是母婴间ＨＣＭＶ感染传播的感染源。ＨＣＭＶ感染对胎儿发育的影响，应引起优生优育工作者们的重视。
A nested polymerase chain reaction(PCR)for detection of human cytomegalovirus(HCMV)and its clinical application to gynecology and obstetrics are reported.Procedures ofDNA separation from clinical samples were simplifled.Conditions of the nested PCR wereoptimized.Amplification multiple of the nested PCR was 2.9 × 10￣9 and sensitivity of the nestedPCR / electrophoretic method was 40 copies of HCMV genome。Clinical results showed that therate of pregnant women suffered from HCMV infection was high. There was a vertical transmiS-sion from the infected woman to her fetus mainly through the placenta and its barrier functionweakened with increase in pregnancy. HCMV infection of a fetus could result from infectedcervical secretion too。It should bring to eugenist′s attention that HCMV infection could endangerthe fetal development.
出处
《军事医学科学院院刊》
CSCD
北大核心
1995年第3期204-208,共5页
Bulletin of the Academy of Military Medical Sciences
关键词
巨细胞病毒
聚合酶链反应
感染
宫内
cytomegalovirus
polymerase chain reaction
infection,intrauterine
gyne-cology
obstetrics
