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甘蔗叶片感染甘蔗花叶病毒后ATPase活性定位和超微结构变化

ULTRASTRUCTURAL LOCALIZATION OF ATPASE ACTIVITY IN THE INFECTED AND NON-INFECTED LEAVES OF SUGARCANE BY SUGARCANE MOSAIC VIRUS
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摘要 研究表明:闽糖70/611,闽选703,F134,福引79/8,福引79/9,NCo310和Co740等7个抗病性不同的品种,受甘蔗花叶病毒感染后叶片叶肉细胞质膜,大、中、小维管束韧皮部筛管和伴胞质膜以及伴胞细胞质中ATPase活性均明显高于其健株相应部位;而维管束鞘细胞质膜ATPase活性则底于其健株相应部位。褪绿斑部位叶肉细胞叶绿体基本解体或消失;富含叶绿体的维管束鞘细胞多为淀粉粒所占据;结果表明染病后ATPase活性的变化程度似乎与品种抗病性强弱有关。 Ultrastructural localizations of ATPase activity in the infected and non- infected leaves by sugarcane mosaic virus str.A(SCMV-A)with 7 sugarcane varieties were compared by a routine enzyme cytochemical technique.Results showed that ATPase activity the cytoplasmic membrane of mesophyll cells, phloem sieve and companion cells in 3 types of bundle and cytoplasm of com- panion cells in SCMV-A-infected leaves with 7 different resistance varieties, i.e.Mintang 70/611,Minxian 703,F 134,Fuyin 79/8,Fuyiu 79/9,NCo 310 and Co 740,was obviously higher than that in the healthy leaves correspon- dingly,whereas ATPase activity in the cytoplasmic membranes of bundle shea- th in SCMV-A-infected leaves was lower than that in the healthy sugarcane leaves.Chlorophylls in the mesophylls with chlorotic lesions were almost dege- nerated or disappeared.Bundle sheath cells with abundant chlorophylls origi- nally were occupied by starch particles after their infection by SCMV-A.The change level of ATFase activity between infected and non-infected leaves seemed to correlate to the resistability and susceptibility of sugarcane variety.
机构地区 福建农学院
出处 《植物病理学报》 CAS CSCD 北大核心 1989年第2期69-73,共5页 Acta Phytopathologica Sinica
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参考文献3

  • 1裘维蕃,植物病毒学,1974年
  • 2彭时尧,植物学报,1988年
  • 3简令成,实验生物学报,1983年,16卷,2期,133页

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