摘要
为提高厌氧发酵的效率,实现沼气发酵的可控化,采用序批次反应器来研究嗜热性微生物菌群在不同温度条件下对不同底物产甲烷潜力的变化规律.玻璃反应器(108 mL)中分别加入8 mL嗜热微生物菌群和0.5 mL的底物(乙酸或丙酸)以及31.5 mL的营养液,然后放置在9个不同处理温度(25～65℃)的生化培养箱中进行培养.反应器中甲烷的产量用气相色谱(Shimadzu GC-8A)来分析和测定.结果表明,当以乙酸作为底物时,该嗜热菌群的甲烷最大生产率在各处理温度都要高于以丙酸作为底物时平行样的产气率;试验所用嗜热菌群的最佳产气温度为40℃和55℃;在低温(≤30℃)和高温(＞60℃)条件下,该菌群表现为极低的产气率,同时表明该菌群对此温度范围极为敏感.
The response of anaerobic conversion of acetate and propionate to changes in temperature is investigated. 8 mL fresh thermophilic inoculum were placed in a glass vial of 108 mL with 0.5 mL substrate （acetate or propionate） and 31.5 mL nutrient solution. The vials wcre incubated at 9 different treatment temperatures ranging from 25 to 65 ℃. The methane formation in the headspace of vials was analyzed by a Shimadzu GC-8A equipped with a flame ionization detector （FID）. The methane production capacities when acetate was used as the substrate were higher than that when propionate was the substrate at all treatment temperatures. The optimum temperatures for this thermophilic inoculum were found at 40 ℃ and 55 ℃. When the temperature is lower than 30 ℃ or higher than 60 ℃, the methane production capacities are sharply decreased, indicating that this thermophilic inoculum is very susceptible to those temperatures. In conclusion, changes in temperature are strongly to have a substantial influence on the methane production capacities. Screening appropriate microbial culture and optimizing treatment temperature is the key for the design of anaerobic digestion in large scale application.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第4期422-426,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南农业大学引进人才基金资助项目(03YJ05)
关键词
温度
嗜热菌
甲烷
乙酸
丙酸
厌氧发酵
temperature
thermophilic inoculum
methane
acetate
propionate
anaerobic conversion
