摘要
本室所建立的小鼠胸腺上皮细胞株ＭＴＥＣＢ以５．０×１０５细胞／ｍｌ的浓度在含１０％小牛血清的ＤＭＥ培养液中，５％ＣＯ２３７℃条件下，培养６ｄ，离心收集上清液。以ＩＬ－６依赖细胞株采用ＭＴＴ法对ＭＴＥＣＢ的上清液进行ＩＬ－６活性检测。结果发现ＭＴＥＣＢ能自发产生低水平的ＩＬ－６，其活性单位为１９．２Ｕ／ｍｌ。ＭＴＥＣＢ的ＩＬ－６产生量低于文献报道的其他胸腺上皮细胞株，说明我们建立的胸腺上皮细胞株有新的特点，反映出胸腺上皮细胞存在高度异质性。
MTECB is a murine thymic epithelial cell line established in our laboratory. MTECB were set up at the initial concentration of 5. 0×105 cells/ml and cultured in the DME containing 10% newborn calf serum. After 6 days, the culture supernatant of MTECs was collected. In our experiment,it is found that MTECB supernatant augments the proliferation of 7TD1 (a IL-6 dependent cell line) ,and the augmentation can be inhibited by IL-6 McAb. This fact indicates that MTECB is able to produce IL-6spontaneously.The IL-6 activity produced by MTECs is on average of 19. 2U/ml and lower than that of the thymic epithelial cell lines reported by other authors.This study further indicates the high heterogeneity of thymic epithelial cells.
出处
《解剖学报》
CAS
CSCD
北大核心
1995年第4期418-421,共4页
Acta Anatomica Sinica
基金
国家自然科学基金
美国洛氏基金
