摘要
目的:分析乳腺癌与正常乳腺组织中癌基因、抗癌基因的差异表达,为乳腺癌发生的分子病因学提供线索。方法:选择288条已知人肿瘤相关基因制备寡核苷酸探针芯片,提取16例乳腺癌与相应正常乳腺组织mRNA,通过逆转录制备荧光标记的cDNA探针并与芯片杂交,经扫描获取图像,计算机分析两组表达差异。Cy3/Cy5>3.5为表达显著性增高;Cy3/Cy5<0.25为表达显著性降低。结果:16例乳腺癌组织与对照组比较,共有84条基因表达存在差异,表达显著性上调的6条,显著性下调的4条。结论:乳腺癌组织与正常乳腺组织相比,存在部分差异表达的基因,乳腺癌的发生与这些基因表达差异密切相关。
Objective： To identify evidence of pathogenesis of breast cancer by analyzing differential expression of the oncogene and antioncogene existing in breast cancer and normal breast tissues. Methods： The related human tumor genes containing 288 genes were chosen for preparation of the oligochips probe, mRNA was extracted respectively from the 16 breast cancer tissues and the corresponding normal breast tissues, and a cDNA probe was prepared through reverse-transcription and hybridized with the gene chip. A laser focused fluorescent scanner was used to scan the chip. The different gene expressions of computer software were thereafter automatically compared and analyzed between the two sample groups. Cy3/CyS〉3.5 meant significant up-regulation. Cy3/CyS〈0.25 meant significant down-regulation. Results： The comparison between the breast cancer tissues and their corresponding normal tissues showed that 84 genes had differential expression in the Chip. Among the differently expressed genes, there were 4 genes with significant down-regulation and 6 with significant up-regulation. Conclusion： Compared with normal breast tissues, the expressed genes do partially exist in breast cancer tissues. These genes of different expression are closely related with the occurrence and evolution of breast cancer.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2005年第15期872-875,共4页
Chinese Journal of Clinical Oncology
关键词
乳腺肿瘤
差异表达基因
基因芯片
Breast neoplasm Differentially expressed genes Gene chip
