摘要
目的探讨提高大鼠原位肝移植手术质量的技巧和注意事项。方法通过改良的双袖套法对115只大鼠进行了原位肝移植(不包括预试验),设定供肝冷保存时间为60min。观察手术成功率和术后生存率以及手术并发症。结果手术成功率为95.6%,1周存活率为88.7%。结论熟练的显微外科技术和缩短无肝期时间是手术成功的关键,采用各项改良措施并注意手术的细节是手术成功的有效保障。
Objective To provide a reliable model of orthotopic liver transplantation in rats and study surgical techniques for improving the quality of the operation. Methods The model was established by modified Kamada＇ s cuff technique. The time of cold preservation was 60 min. The successful rate of the method and one-week survival rate were observed. Results In 135 cases undergoing orthotopic liver transplantation, one-week survival rate was 89. 2%. The successful rate of the model was 95.6%. Conclusions The key for success in rat liver transplantation is the shortened anhepatic period and delicate and skillful surgical manipulation. Also some detailed modified techniques should be used.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2005年第8期500-502,共3页
Chinese Journal of Organ Transplantation
