摘要
研究慢性充血性心力衰竭(简称心衰)时,干细胞移植对心肌电生理学特性的影响。日本大耳白兔分为健康对照(8只)和心衰两大组,后者包括骨髓间充质细胞移植组(8只)、骨骼肌成肌细胞移植组(6只)和假手术组(8只)。慢性心衰模型由阿霉素制成。干细胞移植采用经双球囊封堵主动脉窦上部灌注自体骨髓间充质细胞或骨骼肌成肌细胞。比较干细胞移植前后心功能有无变化。测定多部位的有效不应期(ERP)及离散度(ERPd);右室流出道、左室心尖部或室间隔连续刺激(S1S1)或早搏刺激时,其它各部位的兴奋传导时间(AT)和兴奋传导时间离散度(ATd),比较各组间有无差异。结果:骨髓间充质细胞移植4周后,左室射血分数有改善的趋势。各组间ERP及ERPd无差异。与健康对照组和假手术组相比,骨骼肌成肌细胞移植组和骨髓间充质细胞移植组的AT和ATd有增加,在骨骼肌成肌细胞移植组更加明显。AT和ATd的变化在左室心尖部刺激时最为显著。在骨髓间充质细胞移植组,出现1例自发性室性心动过速,并猝死。结论:慢性充血性心衰时,干细胞自体心脏移植后,心肌组织的电传导能力下降,离散度增大。
In chronic heart failure （HF） models, we investigated whether stem cell transplantation would affect myocardial electrophysiology. Japanese white ears rabbits were divided into health group （ n = 8） and HF group, the latter including bone marrow stromal cells transplantation （MSC） group （n =8）, skeletal myoblasts transplantation （SMs） group （n =6） and sham group （n =8）. HF model was made by adriamycin. Autologous MSC or SMs were introduced into coronary arteies by occluding the root of ascending aorta. After 4 weeks, the electrophysiological properties of transplanted hearts were assessed. In MSC group, the dispersion of effective refractory period had tendency of prolongation. MSC or SMs transplantation caused extension of activation time （AT） and exacerbation of AT dispersion during burst or presystolic pacing. In MSC group, an episode of spontaneous ventricular tachycardia was observed. Conclusion：Abnormal properties of myocardial electrical conduction are rendered by stem cell transplantation.
出处
《中国心脏起搏与心电生理杂志》
2005年第2期124-127,共4页
Chinese Journal of Cardiac Pacing and Electrophysiology
基金
国家自然科学基金资助项目(批准号:30270566)
