摘要
应用八倍体小麦－簇毛麦和普通小麦杂交、回交，试图将其抗白粉病基因导入普通小麦。通过细胞学分析和染色体分带分析证明双二倍体小簇麦含有５６条染色体，其染色体组为ＡＡＢＢＤＤＶＶ，其与普通小麦的杂种Ｆ＿１代减数分裂的中期Ｉ二价体数目常少于２１对，单价体数目常多于７个，并出现三价体、四价体和少量五价体。应用生物素标记的簇毛麦总ＤＮＡ探针对小簇麦杂种进行染色体原位杂交，能够清楚检测出簇毛麦的染色体，双二倍体小簇麦含有１４条簇毛麦染色体，９４００９—５—４含有Ｖ＿２，Ｖ＿３和Ｖ＿４３条簇毛麦染色体，９４００９－５－９含有１条簇毛麦的Ｖ＿６染色体。目前已选育了优良抗病品系，经温室和田间人工接种鉴定，表现高抗小产白粉病。
In an attempt to transfer powdery mildew resistance of Haynaldia villosa(2n=14)intoTriticum aestivum,the amphiploid T.aestivum-H.villosa(AABBDDVV)was used to cross with wheat cul-tivars.The resulting F_1 plants used as female were backcrossed to t he wheat cultivars,and the BC 1 plantswere obtained.The meiotic pairing of PMCs in F_1 plants showed that more than seven univalents were ob-served, and trivalent and quadrivalents were also found in some cells analyzed.C-banding and genomic in situhybridization were used to identify the chromosomes of the BCl-derivedplants, and two plants(94009/5-4and 94009/5-9)were shown to contain H. villosa chromosomes. 94009 /5-4 had three H. villosa chromosomes(2,3 and 4):94009/5-8was found to have one chromosome 6 of H. villosa.In addition, Several wheat lineswith powdery mildew resistance of H. villosa have ben obtained and identified for their resistance in greenhouse and disease nursery field.
出处
《江苏农学院学报》
CSCD
1995年第3期9-14,共6页
Jiangsu Agricultural Research
基金
国家小麦育种攻关课题
国家自然科学基金
关键词
小麦
白粉病
基因导入
抗病性
近缘种属
triticum aestivum
haynaldia villosa
amphiploid
powdery mildew resistance,alien genetransfer
