摘要
从石竹科植物九子参(Silene rubicunda Franch.)根中得到一个糖链上带双乙酰基的新三萜八糖苷—九子参苷A(rubicunoside A,1).经化学降解反应、酶解和波谱分析,得到15个衍生物,其结构最后确定为皂树酸-3-o-β-D-吡喃半乳糖-(1→2)-[-β-D-吡喃木糖-(1→3)]-β-D-吡喃葡萄糖醛酸-28-O-β-D-吡喃木糖-(1→3)-β-D-吡喃木糖-(1→4)-α-L-吡喃鼠李糖-(1→4)-[2"-O-乙酰基-β-D-吡喃鸡纳糖-(1→2)]-[3′-O-乙酰基]-β-D-吡喃夫糖苷{3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl quillaic acid28-O-β-D-xylopyranosyl-(1→3)-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)-[2"-OAc-β-D-quiovopyranosyl-(1→2)]-[3′-OAc]-β-D-fucopyranoside,1}.同时发现甘草酸酶的两个新酶解特性.
A new acetylated triterpenoid saponin, rubicunoside A (1), was isolated from the roots of Silene rubicunda Franch. It was characterized on the basis of extensive chemical, enzymic, and spectral methods as 3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)] -β-D-glucuronopyranosyl quillaic acid 28-O-β-D-xylopyranosyl-(1→3)-β-D-xylopyranosyl-(1 →4)-α-L-rhamnopyranosyl-(1→4)-2'-OAc-β-D-quinovopyranosyl-(1→2)]-[3'-OAc]-β-D-fucopyranoside (1). During the enzymic cleavage with glycyrrhizinic acid hydrolase, we unexpectedly found two new characteristics of the hydrolase. It can selectively hydrolyzed not only 28-O-monoprosapogenins, but also the acetyl group in the sugar moieties of the prosapogenins. The enzymic cleavage method with glycyrrhizinic acid hydrolase providing selectivly 3-oligosaccharides and 28-O-glucosides and partial methanolysis providing selectively 28-oligosaccharides from 3,28-O-bisglucosides were effective for the structure elucidation.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
1995年第10期1024-1033,共10页
Acta Chimica Sinica
基金
国家自然科学基金
中国科学院昆明植物研究所植物化学开放实验室资助的项目.
