摘要
研究了细胞生长过程中微载体的种类、浓度及细胞接种量对培养Ｐｘ细胞的影响．以ＧＴ－３为微载体，当细胞接种量为２．０×１０５细胞／ｍＬ，微载体浓度为２．０ｍｇ／ｍＬ，温度为２８℃时，第６天细胞密度可达１．０×１０６细胞／ｍＬ，为接种量的５倍，细胞生长旺盛，形态正常．
Several factors which may affect the growth of Px cell have been studied. The kind and concentration of microcarriers,cell inoculation density can influence cell growth substantially. Under the following conditions: GT-3 microcarrierconcentration, 2. 0 mg/mL ;cell inoculation density, 2. 0×105cells/mL ;and temperature 28℃,cell concentration can reach 1. 0×106 cells/mL after 6 days culture. Thehighest cell concentration is almost 5 times as high as the initial cell inoculationdensity. The cell on microcarriers were grown satisfactorily.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
1995年第1期96-99,共4页
Journal of Central China Normal University：Natural Sciences
