摘要
用小菜蛾颗粒体病毒［ＰｌｕｔｅｌｌａｘｙｌｏｓｔｅｌｌａｇｒａｎｕｌｏｓｉｓＶｉｒｕｓ（ＰｘＧＶ）］侵染家蚕胚胎细胞系Ｂｍ－２１Ｅ－ＨＮＵ５，对受染细胞的超薄切片微电镜观察，在攻毒后１２０ｈ的受染细胞核内开始发现病毒发生基质、核衣壳和病毒粒子；病毒粒子以出芽方式通过细胞核膜排出核外；攻毒后１４４ｈ蒴状体所在的囊膜和细胞核中可见大量小膜泡．
Established cell line Bm-21E-HNUS,from embryos of Bombyx mori,wasinfected with Plutella xylostella granulosis virus (PxGV). Transmmission electronmicroscopic examination of cell cultures in feted showed virogenic stroma and nucleocapsids and enveloped nucleocapsids in nuclei 5 days pi. The vinous replicated escaped the nuclei by budding through the nuclear membrane. Many capsules werefound in some special vacuoles in cytoplasma 6 days pi. There were many miniamvesicles in both the nuclei infected and the vacuoles containing capsules.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
1995年第1期100-103,共4页
Journal of Central China Normal University：Natural Sciences
关键词
颗粒体病毒
小菜蛾
形态发生
离体细胞
Plutella xylostella
granulosis virus
in vitro replication
Bombyx moricell line
