摘要
用孔雀绿比色法可测出０．１μｇ磷，重现性好，变异系数为２．０３％，回收率为９４．４％～１０３．４％，显色稳定，操作简便。红细胞膜Ｃａ￣（２＋）－ＡＴＰ酶和Ｎａ￣＋、Ｋ￣＋－ＡＴＰ酶经低渗皂素溶血后，在Ｃａ￣（２＋）、ＥＧＴＡ或哇巴因的存在下，水解ＡＴＰ释出的无机磷，用孔雀绿法可分别测出其酶活性。
Activities of Ca￣(2+)-ATPase and Na￣+,K￣+ -ATPase of hemolysate were simultaneously deter-mined by a colorimetric method with malachite green dye. In the presence of Ca￣(2+),EGTA or ouabain,inor-ganic phosphate released from ATP by hypotonic saponin-treated red cell lysate was used to estimate theactivities of Ca￣(2+)-ATPase and Na￣(2+),K￣+ -ATPase. Themethod is simple,sensitive and stable for at least 4h.The effects of concentration of saponin and Ca￣(2+),thestorage of hemolysate,and the activator and inhibitorof the enzymes on activity were discussed.
出处
《华西医科大学学报》
CSCD
1995年第2期230-233,共4页
Journal of West China University of Medical Sciences
关键词
孔雀绿比色法
溶血液
腺苷三磷酸酶
钙
钾
钠
Ca￣(2+)-ATPase Na￣+
K￣+ -ATPase Hemolysate Malachite green colorimetric assay
