摘要
采用硫酸铵分段盐析，肝素－葡聚糖亲和层析和ＤＥＡＥ－交联葡聚糖Ａ－５０离子交换层析等方法从人血浆中分离纯化出富含组氨醚糖蛋白，经双向免疫扩散和ＳＤＳ－ＰＡＧＥ鉴定，表明待鉴定样品为单一区带、分子量６６Ｋｄ的富含组氨醚糖蛋白纯品。富含组氨醚糖蛋白通过同ＡＴ－Ⅲ竞争肝素结合而具有较强的促凝活性，其同肝素间的相互作用依赖于Ｃａ２＋的存在，ＥＤＴＡ，枸椽酸盐对此具有浓度依赖性的抑制作用。
n this paper. rnethods of ammonium sulfate fraction, heparin
sepharose affinity andDEAE-Sephadex A-50 chromatography were used to isolate and purify
histidine-rich gly-coprotein (HRG) from fresh human plasma. Analyzed by double
immunodiffusion and SDS- PAGE , the purified sample produced a single precipitin arc when
diffused against standardantiserum to human HRG. It showed a single band with an apparent
molecular weight of66Kda, which was identified as HRG. The experimental studies revealed
that HRG couldinhibit the anticoagulative activity of AT- Ⅲ and hence shorten the thrombin
time. This ac-tivity depends on the presence of calcium ion and can be counteracted by calcium
ion bindinganticoagulants such as EDTA and citrate. EDTA and citrate inhibit the interaction
betweenHRG and heparin which have concentration dependent relationship.
出处
《湖南医科大学学报》
CSCD
1995年第4期315-318,共4页
Bulletin of Hunan Medical University
基金
国家自然科学基金
关键词
组氨酸
糖蛋白
纯化
血浆
促凝活性
histidinerich glycoprotein (HRG) 3 purificationi activityi plasma
human
