摘要
用琼脂糖凝胶电泳法测定了斜纹夜蛾核型多角体病毒（ＳＩＮＰＶ）经６０Ｃｏγ射线辐照产生的ＤＮＡ单链和双链断裂频数，得到在１－１００ｋＧｙ剂量范围内，ＤＮＡ单、双链断裂频数与吸收剂量的关系。实验结果表明，ＳＩＮＰＶ－ＤＮＡ对γ辐射的链断裂响应可以用单靶一次击中和二次击中复合模型描述，单链断裂是一次击中效应，而病毒ＤＮＡ的双链断裂是一次击中和二次击中事件的共同效应，其中以二次击中事件效应为主。
The Spodoplera litura Nuclear Polyhedrosis Viruses (SINPV)is a kind of insectile virus with a simple structure, in which a double helix DNA is encapsulated in a protein coat and there is no function of enzymatic repair. In this study, the SINPV samples in dry powdered form held in sealed plastic tube were irradiated by 1-100kGy gamma rays. The single strand breaks (SSB) and double strand breaks (DSB) induced in SINPV after irradiation were measured by neutral and alkaline agarose gel eletrophoresis.A dose-response function combining the responses of one-hit and two-hit events was used to describe the SSB and DSB dose-response curves. It is shown that the SSB are one-hit events and the DSB are the combination of both one-hit, and two-hit events, and two-hit events are predominant in the DSB process.
出处
《核技术》
CAS
CSCD
北大核心
1995年第7期405-409,共5页
Nuclear Techniques
基金
国家自然科学基金
关键词
斜纹夜蛾核
多角体病毒
DNA链断裂
辐射剂量
Spodoplera litura Nuclear Polyhedrosis Viruses(SINPV),DNA strand breaks, Dose response function
