摘要
为探索空间条件对植物种子的诱变作用，对粳稻“农垦５８”纯系单株种子经“８８８５”卫星搭载处理后在地面种植，筛选出已稳定遗传的大粒型突变系，对其基因组的突变位点进行了分子生物学分析，运用１４０个随机引物对“农垦５８”原种及其大粒型突变系进行多态性研究，在扩增的２０００多条ＤＮＡ片段中，仅有５个片段显示了多态性，即所检测的２０００个染色体位点上仅５个位点有差异，占０．２５％，其中４个片段为重复序列，一个片段为２个拷贝序列。应用水稻窄叶青８号和京系１７杂交Ｆ１代花药培养ＤＨ群体为作图群体，将该片段定位于第１１号染色体上，位于分子标记ＣＤＯ５２０和ＰＴＡ８１８中间。
In order to investigate the mutation effects of outer space condition on plant
seeds,the cul-tivated rice“Nongken 58”seeds were carried into outer space by the
recoverable satellite“ 8885”in1988.The returned seeds were planted in the fields in Hainan
province. Large-grained mutant wasobserved in SP2 generation and selected. The mutant was
geneticly stable through six generations byself-cross. Molecular identification of the mutant has
been conducted by RAPD analysis, only five ofthe used 140 random 10-mer primers amplified
polymorphic DNA fragments between“Nongken 58”and its large-grained mutant.The
polymorphism degree is 0.25%. These polymorphic fragmentswere proved to be repeated
sequences except OPA18-3, which have only two copies in rice genome.RFLP mapping was
performed with a DH population from ZYQ8/JX17.Its location is on chromosome11 between the
markers CDO 520 and PTA 818. The sequence of OPA18-3 was also analysed.
出处
《航天医学与医学工程》
CAS
CSCD
1995年第2期109-112,共4页
Space Medicine & Medical Engineering
基金
国家高技术计划
联合国能源组织资助
关键词
科学卫星
空间实验
水稻
种子
突变体
基因组
scientific satellites spaceborne experiments rice seeds mutation line genomechromosome
translocation RAPD analvsisAddress reprint requests to:XING Jinpeng.Institute of
Genetics,Academia Sinica, Beijng 100101,China
