摘要
本文报告了用逆转录套式多聚酶链式反应(RT nested PCR)和酶联免疫吸附试验(ELISA)检测血清中HCV—RNA和抗—HCV.用军科院丙肝抗体酶免试剂盒检测了2086份血清,病区和门诊病人抗—HCV阳性率为13.4%,献血员为2.2%,一些高危人群HCV感染率比较高.用军科院和肝研所两个单位的丙肝抗体酶免疫试剂盒同时检测100份血清,抗—HCV总符合率为95.0%.用PCR法检测了34份抗—HCV阳性和20份抗—HCV阴性血清,阳性符合率为70.6%,总符合率为79.6%.PCR与ELISA法检测HCV感染各有长短,目前仍需找到一种敏感、特异、简便、快速的诊断方法,以适应临床需要.
KT 'nestd'PCR and ELISA were used to detect serum HCV-RNA and anti-HCV. Among 2086 samples, the positive rate of anti-HCV in patients was 13.4%. and in donors 2.2% (Academy of Military Medical Science, HCV ELISA Kit)). The coincidence rate of anti-HCV detected simultaneously by HCV ELISA kit 0f Academy of Military Medical Science and Institute of viral Hepatitis, Beijing Medical University in 100 samples was 95.0%. Serum samprs inchiding 34 positive and 20 negative anti-HCV were detected by PCR. Positive coincidence ratewas 70.6% and total coincidence rate was 79. 6%. It is showed bath PCR and ELISA had virtuesand defects in detecting HCV infection. A sensitive, specific. simple and quick method is still awaiting to be found.
出处
《海军总医院学报》
1995年第1期20-23,共4页
Journal of Naval General Hospital of PLA
关键词
丙型肝炎病毒
感染
聚合酶链反应
ELISA
:Polymerase Chain Reaction Enzyme-linked Immunosorbent Assay Ribonucleic acid Antibodies to hepatitis C Virus
