摘要
从广西各地的八个温泉的水样中分离到２０株耐药的嗜热脂肪芽孢杆菌，用改良的ＢｉｎｇｈａｍＳＤＳ裂解法，从其中的１５株菌中检测到质粒。再用琼脂糖凝胶电泳法及电子显微镜技术对耐氨苄青霉素（２５μｇ·ｍｌ－１）的１２号菌株的质地作了较深入的研究。发现该质粒为共价闭合坏状ＤＮＡ分子（ＣＣＣＤＮＡ），共分子量为１４．６３×１０６，澳化乙锭（１μｇ·ｍｌ－１）和吖啶橙（２０μｇ·ｍｌ－１）共同作用可将该质粒消除掉。该质粒的功能有待进一步研究。
Twenty a ntibiotic-resistant strains of Bacillus Stearothermophilus isoltatedfrom eight hot springs of Guangxi were exilmined for the pressence of plasmids by theBingham's modified method. Fifteen of 20 strains were found. to contain a singleplasmid. Through analysis by agarose-gel electrophoresis and electron microscopy, theampicillin-resistant Baeillus stearothermophihilus 12-was found to contain a plasmid ofmolecular weight 14.63 × 106(21 .95 kb). This plasmid is a covalently closed circular DNAmolecule (CCCDNA). It can be cured by ethidium bromide plus acridine orange. Its function isn't known. It's a cryptic plasmid.
出处
《广西大学学报（自然科学版）》
CAS
CSCD
1995年第2期125-129,共5页
Journal of Guangxi University（Natural Science Edition）
关键词
芽孢杆菌
质粒
检测
消除
嗜热菌
Bacillus stearothermophilus
plasmid
detection
curing
