摘要
用ELISA双夹心法、病毒分离和IFA法,平行检测了72例急性下呼吸道感染患儿鼻咽分泌物中呼吸道合胞病毒(RSV)抗原,结果诊断RSV感染的阳性率分别为72.2％(52例)、81.9％(53例)和81.1％(44例)。同病毒分离比较,ELISA的敏感性、特异性和诊断符合率分别为86.4％,92.3％和87.57％,IFA的敏感性、特异性和诊断符合率分别为74.6％、100.0％和79.2％。患儿年蛉,病情和标本采集时间等因素,对ELISA敏感性有一定影响。ELISA检测RSV抗原特异、敏感、快速(收到标本后7 h内可获得结果),适用于婴幼儿,尤其9月龄以内小儿RSV感染的早期诊断。
An enzyme-linked immunosorbent assay (ELISA) for the rapid diagnosis of respiratory syncytial virus (RSV) infection by means of demonstration of viral antigens directly in the specimens was developed and was compared with tissue culture virus isolation (TC) and indirect immunofluorescence-antibody test (IFA). Nasopharyn-geal secretions (NPS) from 72 patients hospitalized with acute lower respiratory tract infection were obtained during the period from January to April, 1988. The patients were 11 days to 28 months old with 73.6% of them under 1 year of age. Of 72 samples screened in parallel by all three methods, 59(81.9%), 52(72.2%) and 44(61.1%) were postive for RSV by TC, ELISA and IFA, respectively. Sensitivity, specificity and accuracy by ELISA were 84.6%, 92.3% and 88.1%, respectively, but 74.6%, 100% and 79.2% by IFA compared with TC. The ELISA could be easily performed within 7 hours. The described ELISA is capable of detecting ficollpurified RSV antigens at protein concentration of 10 ng/ml. It is shown that the ELISA is a sensitive and specific method for. the detection of RSV antigen in NPS and that the ELISA may be applied to the rapid diagnosis of RSV infection of children, especially the infants younger than 9 months old.
出处
《第四军医大学学报》
1989年第5期323-327,共5页
Journal of the Fourth Military Medical University
关键词
RSV
感染
儿童
ELISA
诊断
enzyme-linked immunosorbent assay
diagnosis
respiratory syncytial viruses
infection
