摘要
造血组织是辐射高敏感组织之一，胚胎动物的造血组织对辐射更为敏感。本文观察了持续性小剂量率γ射线照射妊娠中期母鼠，对出生子鼠股骨骨髓有核细胞数（ＢＭＣ）、造血干细胞（ＣＦＵ－Ｓ）数及其在股骨骨髓腔中分布状况的改变，和对造血组织基质细胞（ＣＦＵ－Ｆ）的变化。本实验观察到正常小鼠股骨骨髓ＣＦＵ－Ｓ分布，接近骨髓骨皮质的ＣＦＵ－Ｓ浓度最高，约为４１／１０５ＢＭＣ，近骨髓中心静脉窦ＣＦＵ－Ｓ浓度最低，大约是２２／１０５ＢＭＣ。６０Ｃｏγ射线从妊娠１３ｄ到出生前持续性照射６ｄ，分为１５ｃＧｙ／ｄ剂量组和６０ｃＧｙ／ｄ剂量组，出生子鼠成长到８周龄时，可见到股骨骨髓中ＣＦＵ－Ｓ的分布紊乱，特别是在接近骨髓皮质部分的ＣＦＵ－Ｓ数量明显下降，股骨骨髓中ＣＦＵ－Ｓ总数也明显下降，１５ｃＧｙ／ｄ组下降了２０％，６０ｃＧｙ／ｄ组下降了３３％，骨髓基质中ＣＦＵ－Ｆ数量在１５ｃＧｙ／ｄ组下降１２％，而６０ｃＧｙ／ｄ组下降３３％。统计学处理均有显著性差异。
The radial distribution of CFU-S in the femur of a normal 8 weeksold mouse is shown and corresponds closely to Lord and Hendry. The highest concentration (about 41/105 cells) was near to the bone surface and the lowest (about22/105 cells)was close to the central venous sinus which runs roughly along the longitudinal axis of the bone shaft. Continuous exposure to γ-rays for 6 d from 13dof gestation to birth had a smaller effect on the total femoral cellularity at 8 weeksof age, decreasing it by 12%-20%. The 15 cGy/d regimen caused a significant(20% ) CFU-S deficit (p<0.02) in the marginal zones. The 60 cGy/d scheduleincreased the deficit to a level (33%) and the change in the femoral CFU-S distribution after γ-ray irradiation is also shown. The 60 cGy/d schedule also reducedthe fibroblastoid, CFU-F POpulation, to 67% of control (P<0 .001). The 15 cGy/dreduced to 88% of control.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
1995年第2期118-122,共5页
Journal of Radiation Research and Radiation Processing
关键词
妊娠鼠
造血干细胞
子鼠
Γ辐射
Pregnant mice
OffSpring of mice
γ-irradiation
